In addition to the standard baking pad length of 10 mm, we also offer pad lengths ranging from 1 mm to 100 mm. Light paths smaller than 1 mm are also available, but are for more specialized cells, such as detachable buckets or HPLC flow buckets. Mix the sample well before use, especially when nucleic acids or proteins are stopped again. Avoid using glass pipettes to fill the bucket, as these can cause scratches on the optical surface.
Extra care should be taken to avoid touching the windows after the cleaning is complete. The sample should be removed immediately after measurement to prevent damage to the bucket by solvent evaporation. The cuvette used to measure uv cuvettes a sample is part of the spectrophotometer’s optical system. Therefore, the position, geometry and condition of the bucket can affect the accuracy and precision of absorption measurements and should be carefully monitored.
The material in the bucket can cause its own absorption, depending on the wavelength. Quartz semimers are fully translucent over the entire measuring range (UV/Fish). Disposable plastic buckets are often only translucent in the visible range of the spectrum. Therefore, the selection of the bucket depends mainly on the application. The decision on which type of bucket to choose depends on the instrument used, the nature of the application and the properties of the sample. In general, it is important that the cuvettes are as transparent as possible so that the wavelengths are measured so as not to limit the available linear range of the photometer.
You are on the bench and need to perform a specific type of measurement with your specific Zetasizer model… But there are many options and you are not sure which bucket is most suitable. You want to have confidence in your choice and in the possibilities of both your bucket and the instrument.
Buckets with a length over long paths with optical path lengths of 20 mm, 30 mm, 40 mm, 50 mm and 100 mm are available. The absorption increases proportionally with the length of the optical path of the buckets. Fluorescence buckets are buckets with 4 light walls, while absorption buckets are usually 2 clear-walled ones. Cuvettes f and g are examples of non-standard size cuvettes, which are also called short-path length cuvettes. The length of the pad and the external size are smaller than standard buckets. This consideration is especially important for buckets that are designed to measure small volumes and therefore have very small measuring windows.
Since plastic buckets are used only once and do not need to be cleaned, there is no need to take into account possible damage and loss. They are an excellent choice for fake hellometry and spectrophotometry. There is a negligible variation in absorption between the four optically bright sides, so that the measurement can be taken from both sides.
All these materials seem absolutely clear and suitable for all kinds of absorption studies at first glance. However, each material has different light-absorbing properties and it is vital to take these optical characteristics into account when choosing a bucket material. The most popular UV fish buckets refer to buckets with rectangular test tubes, made of quartz material, for use in UV visspectroscopic measuring systems. They are used, like standard test tubes, to store aqueous solutions.
For example, if a photometer with a linear measuring range up to 2 A with a path length of 10 mm is used, the double-stranded DNA can be reliably quantified to a maximum concentration of 100 μg/ml. Solutions of higher concentrations must be diluted or dilution can be simulated using a cuvette with a shorter path length. According to Lambert-Beer’s law, a path length of 1 mm makes it possible to measure dsDNA concentrations up to 1,000 μg/ml. If only a small amount of sample is available, reuse of the sample for later measurements may be considered.
When working on IR spectra, especially for wavelengths greater than 2000 nm, we recommend IR quartz glass buckets because they provide high transmission for these wavelengths.
Plastic buckets, if packaged separately and of an appropriate degree of purity, will minimize the risk of contamination. Alternatively, buckets designed to handle extremely small volumes can be selected. If the sample is based on an aqueous solution, then the material from which the bucket is made is relatively insignificant. If, on the other hand, they are organic solvents, glass buckets have the preferred option, as they show greater strength compared to variants of plastic. For these reasons, certain buckets, such as macro buckets, offer an advantage in temperature-controlled applications.